The peptide reconstitution manual

What's in the vial, and how much water it needs.

Your vial came with a few milligrams of powder and no instructions. Pick it below. We'll show you what's inside, then work out how much bacteriostatic water to add — the amount that makes your dose land on a line you can actually see on the syringe. No shop, no vendor list, no email wall, no account.

Step one

Pick the vial, get the water.

Peptide reconstitution calculator →

Peptide

Amount per vial

Standard size

Target quantity per draw · GHK-Cu

Each button splits the vial into that many equal draws

These buttons are just division. Each one splits the vial into equal draws. They are not a suggestion of how much to take.

Empty on purpose. We don’t pick an amount for anyone, so nothing is pre-selected. Tap a button above and the vial and the syringe fill in.

Open the full Glow calculatorThe full page adds dates, a calendar file and a link you can send

What's covered

Blends first — that's what's in the vial.

All blends →
KLOW80 mg
  • GHK-Cu50 mg
  • KPV10 mg
  • BPC-15710 mg
  • TB-50010 mg

3/4 sourced · KPV missing

Glow70 mg
  • GHK-Cu50 mg
  • BPC-15710 mg
  • TB-50010 mg

3/3 solubility sourced

  • BPC-15710 mg
  • TB-50010 mg

2/2 solubility sourced

Components

each has its own page

Not live yet

  • Weight loss

    Retatrutide · Tirzepatide · Semaglutide · Cagrilintide

  • Growth hormone

    CJC-1295 · Ipamorelin · Sermorelin · Tesamorelin

  • Other

    PT-141 · Melanotan II · MOTS-c · Epitalon

A peptide gets a page once we know what is in it and can source a solubility figure for every ingredient. Not before.

Common questions

Answered with real numbers.

All guides →
How much bacteriostatic water should I add to a peptide vial?

There is no one right amount. It depends on how much you want per draw and which syringe you own. The water decides the strength, and the strength decides which line on the syringe your draw lands on. Here is a real example. A 70 mg Glow vial holds 50 mg of GHK-Cu. Add 2.50 mL and you get 20 mg/mL. So a 2 mg draw is 0.10 mL, which is exactly 10 units on a U-100 syringe — a printed line you can see. Add 3 mL instead and the same 2 mg draw becomes 12 units. Both are correct. One is easier to read. That is why our calculators work out the water for you instead of asking you for it.

Open a calculator
How do I work out the concentration after reconstitution?

Divide the mg in the vial by the mL of water. A 5 mg vial with 2 mL of bacteriostatic water is 5 ÷ 2 = 2.5 mg/mL, which is the same as 2,500 mcg/mL. For a blend, do each ingredient separately. A 70 mg Glow vial holds GHK-Cu 50 mg, BPC-157 10 mg and TB-500 10 mg. Add 2.5 mL of water and you get 20 mg/mL, 4 mg/mL and 4 mg/mL. Every draw takes all three, always in that same ratio.

What's in Glow
How do I convert milliliters to insulin syringe units?

A U-100 syringe splits each milliliter into 100 units. So 1 unit = 0.01 mL, 10 units = 0.1 mL, and 100 units fills it to 1 mL. A U-40 syringe uses a different scale: 40 units per milliliter, so one unit is 0.025 mL. If a number was worked out for U-100 and you draw to "20 units" on a U-40 barrel, you get two and a half times as much liquid. Check which one you have first.

Reading syringe units
What is in KLOW, and how is it different from Glow?

KLOW is an 80 mg blend: GHK-Cu 50 mg, KPV 10 mg, BPC-157 10 mg, TB-500 10 mg. Glow is the same thing minus the KPV, so 70 mg total: GHK-Cu 50 mg, BPC-157 10 mg, TB-500 10 mg. The K in KLOW is the KPV. Neither is a branded product. They are community recipes, so the ratio varies between suppliers. These are the numbers that matched across every source we checked.

KLOW vs Glow, side by side
Does bacteriostatic water need to be refrigerated?

No, not before you open it. An unopened vial keeps at room temperature; follow its own label. The benzyl alcohol in it is what stops bacteria growing, and that is what lets you puncture the vial more than once. What needs the fridge (2–8°C) is the peptide after you have mixed it. We could not find a source for how long a mixed vial keeps in a kitchen fridge, so we do not print a number. Check your product label.

Storing a mixed vial
Do you recommend a quantity or a schedule?

No. The calculators only convert the units and volumes you type in. Nothing is filled in for you and nothing is pre-selected. The preset buttons are just division: each one splits the vial into equal draws. When we cannot find a source for a number, we switch off the feature that needs it and say why on the page. We would rather leave a gap than show a number we cannot back up.

How do I reconstitute a vial without ruining it?

Work out the water before you open anything. Once it is in, you cannot take it back out. Then: let the vial warm up to room temperature. Wipe both rubber tops with a fresh alcohol swab and let them air dry. Draw up your measured bacteriostatic water. Tilt the needle so the water runs down the inside of the glass instead of firing straight onto the powder. Let it dissolve on its own, and roll or swirl the vial if anything is left. Never shake it — shaking foams the liquid and breaks the peptide apart. Anything with GHK-Cu in it turns blue. That is the copper, not contamination.

Full step-by-step
How long does a mixed vial keep?

We do not print a number, and that is on purpose. Suppliers do publish stability data — say six months at −80°C, one month at −20°C. But those are lab freezer numbers. They tell you nothing about a vial sitting at 2–8°C in a kitchen fridge, which is what actually happens. Other sites all repeat 28 days. We went looking for where that number comes from and could not find a source that applies here. Keep it cold, keep it dark, and go by the shelf life your supplier gives you.

Why that number is missing
Can peptides sit at room temperature?

Sealed powder handles room temperature fine. It ships without cold packs and spends days in transit. Once water is in the vial you are asking a different question, because liquid is the least stable state the peptide will ever be in. The thing to actually worry about is not a warm room, it is heat: a parked car, a sunny windowsill, a radiator, hot water, a microwave. Room temperature and heat are not the same thing.

Powder, solution and heat
Which syringe should I use — 0.3, 0.5 or 1 mL?

A smaller syringe does not mean a smaller dose. It means an easier one to read. A 0.3 mL U-100 syringe holds 30 units and prints a line for every single unit. A 1 mL holds 100 units and is usually only numbered every ten. If your answer comes out at 4 units, that is hard to see on a 1 mL barrel and easy on a 0.3 mL. The calculator asks which one you own because the answer changes with it, and it looks for water amounts that land your draw on a line that is really there.

How the barrel is graduated

The papers

What the research actually studied.

Every paper below is real, and you can open it on PubMed yourself. We say what each one tested, not what it might mean for you. And none of them tested a blend. They tested single ingredients, mostly in animals.

  1. Mendias CL, Awan TM2026Sports MedicineReview

    Safety and Efficacy of Approved and Unapproved Peptide Therapies for Musculoskeletal Injuries and Athletic Performance

    Reviews the pharmacology, safety profile and regulatory status of twelve peptides sold direct to patients — BPC-157, GHK-Cu and TB-500 among them. Describes the market in unapproved compounds as operating largely outside regulatory oversight.

    GHK-CuBPC-157TB-500PMID 41966639 →
  2. Vasireddi N, Hahamyan H, Salata MJ, et al.2025HSS JournalSystematic review

    Emerging Use of BPC-157 in Orthopaedic Sports Medicine: A Systematic Review

    Systematic review of the BPC-157 literature published up to 3 June 2024, drawn from PubMed, Cochrane and Embase, covering mechanism, metabolism and safety. States that BPC-157 has no FDA approval and is banned in professional sport.

  3. Seiwerth S, Milavic M, Vukojevic J, et al.2021Frontiers in PharmacologyReview

    Stable Gastric Pentadecapeptide BPC 157 and Wound Healing

    Reviews BPC-157 across skin wound models — incisional and excisional wounds, deep burns, diabetic ulcers and alkali burns — and notes the peptide had previously been used in ulcerative colitis and multiple sclerosis trials.

  4. Siméon A, Wegrowski Y, Bontemps Y, Maquart FX2000Journal of Investigative DermatologyAnimal study

    Expression of glycosaminoglycans and small proteoglycans in wounds: modulation by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu(2+)

    Rat experimental wound model plus rat dermal fibroblast cultures. Repeated 2 mg injections of GHK-Cu raised wound-tissue dry weight and total protein, and increased type I collagen and glycosaminoglycan production.

  5. Hannappel E2007Annals of the New York Academy of SciencesReview

    beta-Thymosins

    Traces what thymosin beta-4 actually is. Identified in 1990 as the main intracellular G-actin-sequestering peptide in most vertebrate cells. TB-500 is a fragment of this protein, not a separate molecule.

  6. Xiao B, Xu Z, Viennois E, et al.2017Molecular TherapyAnimal study

    Orally Targeted Delivery of Tripeptide KPV via Hyaluronic Acid-Functionalized Nanoparticles Efficiently Alleviates Ulcerative Colitis

    Loaded KPV into hyaluronic-acid-functionalised polymeric nanoparticles and delivered it orally in a colitis model. KPV is a naturally occurring tripeptide — lysine, proline, valine.

Every figure this site uses

3 sourced · 5 consensus · 2 missing

How we grade them →

Figures marked consensus are what the community agrees on. We show them, but we never calculate with them. If a figure is missing, we switch off the feature that needed it.

FigureValueGrade
GHK-Cu solubility in waterMedChemExpress product page — Cross-checked: MW 401.91 → 124.41 mM, matches the stated molarity.50 mg/mLSupplier page
BPC-157 solubility in waterMedChemExpress product page — Cross-checked: MW 1419.54 → 70.45 mM, matches. A search snippet claiming 2.5 mg/mL was wrong by 40×.≥ 100 mg/mLSupplier page
TB-500 solubility in waterMedChemExpress product page (opened 2026-08-20) — Cross-checked: MW 889.01 → 224.97 mM, matches exactly. Upgraded from a search snippet.≥ 200 mg/mLSupplier page
KPV solubilityCayman Chemical product page 45458 (opened 2026-08-20) — A qualitative band in PBS, not a measured maximum in water. Not used in any calculation — the low end implies more water than the vial holds. Automatic water volume stays off for KLOW.1–10 mg/mLCommunity consensus
Glow compositionFive independent community sources agreed — Community blends are not branded products; ratios vary by supplier.70 mg — GHK-Cu 50 / BPC-157 10 / TB-500 10Community consensus
KLOW compositionThree independent community sources agreed — Arithmetically self-consistent: 50 + 10 + 10 + 10 = 80 mg.80 mg — GHK-Cu 50 / KPV 10 / BPC-157 10 / TB-500 10Community consensus
Wolverine compositionCommunity sources, 1:1 ratio — The weakest of the three. Some suppliers label a 10 mg vial as 5 + 5.20 mg — BPC-157 10 / TB-500 10Community consensus
Storage temperature after mixingCommunity practice, consistent across sources — GHK-Cu is light sensitive, so Glow and KLOW should be kept dark.2–8 °C, protected from lightCommunity consensus
Shelf life after mixing (beyond-use date)Not found for domestic-fridge conditions — Suppliers publish −80 °C / −20 °C freezer figures only. Consequence: no expiry date anywhere on this site.—Not sourced
Maximum volume per drawNot found — Consequence: the volume filter is skipped. Draws over 1 mL are flagged, never blocked.—Not sourced

Disclaimer

The compounds on this site are research chemicals, not approved medicines. Nothing here is medical advice. Nothing here is meant to diagnose, treat, cure or prevent any condition. Using this site does not make us your clinician. The calculators do one thing: convert the units and volumes you type in. They are measuring tools, not dosing guidance, and they never recommend a quantity, a schedule or a compound to anyone. Talk to a licensed healthcare provider before you make any health decision.

Found a source we missed? Send it. That is the whole point of listing them.